Crystallization (Comment) | Organism |
---|---|
homology modeling of structure, the substrate channel and catalytic pocket are more basic than other NapA proteins, which may influence the substrate binding as well as product release | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
C176S | mutation in strictly conserved residue, significantly lower activity than wild-type | Campylobacter jejuni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
nitrate | wild-type, pH 7.0, 25°C | Campylobacter jejuni | |
0.307 | - |
nitrate | mutant C176S, pH 7.0, 25°C | Campylobacter jejuni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Campylobacter jejuni | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe:Mo ratio is slightly higher than the theoretical value of 4:1 | Campylobacter jejuni | |
Molybdenum | 92% Mo incorporation in active CNapA. The Fe:Mo ratio is slightly higher than the theoretical value of 4:1 | Campylobacter jejuni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | Q5HV12 | - |
- |
Campylobacter jejuni RM1221 | Q5HV12 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrate + reduced methyl viologen | - |
Campylobacter jejuni | nitrite + oxidized methyl viologen + H2O | - |
? | |
nitrate + reduced methyl viologen | - |
Campylobacter jejuni RM1221 | nitrite + oxidized methyl viologen + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NapA | - |
Campylobacter jejuni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.045 | - |
nitrate | mutant C176S, pH 7.0, 25°C | Campylobacter jejuni | |
5.91 | - |
nitrate | wild-type, pH 7.0, 25°C | Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
[4Fe-4S]-center | - |
Campylobacter jejuni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1740 | - |
nitrate | wild-type, pH 7.0, 25°C | Campylobacter jejuni |